Chloroquine gene transfection

Discussion in 'Online Canadian Pharmacy' started by kosarevant, 03-Mar-2020.

  1. Killer Moderator

    Chloroquine gene transfection


    Detailed information on how Wiley uses cookies can be found in our Privacy Policy. This protocol describes a general method for transfecting mammalian cells using linear polyethylenimine.

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    Transfection Methods Reagent-Based Methods DEAE-Dextran Method Overview Solution A DNA ~1–5 µg/ml diluted into 2 ml of growth medium with serum containing chloroquine Solution B DEAE-dextran solution ~50–500 µg/ml Solution C ~5 ml of DMSO Solution D Complete growth medium 1 Add solution A to solution B, then mix gently. Add DNA/CaCl2-solution to 2x HBS drop wise, while blowing air through HBS with pasteur pipette. • Incubate the mixture for 10 to 20min at room temperature. • Remove old medium from the cells. • Add 10ml medium including 25µM Chloroquine the Chloroquine is used in this step only. Pro-Tip* There can be batch to batch variation when making the PEI working stock, therefore the ratio of μg DNAμg PEI needs to be empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1-2 days after transfection to determine what ratio gives the highest percentage of GFP positive cells.

    We specifically use this protocol with Lenti-X 293T cells, a cell line optimized for production of lentiviral vectors. Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short term studies of protein function.

    Chloroquine gene transfection

    Transfection - Wikipedia, Transfection of 293T cells - Lentiviral Gene Ontology Vectors

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  6. Chloroquine is a member of quinolone family and is a weak intercalating agent. Chloroquine is used for treating amebiasis, rheumatoid arthritis, discoid and systemic lupus erythematosus. Application DNA intercalator. Also used to increase transfection efficiency. Chloroquine diphosphate salt has been used • in in vitro antiplasmodial assays

    • Chloroquine diphosphate salt powder or crystals, 98.5-101..
    • Addgene Lentivirus Production Protocol.
    • Enhanced plasmid DNA transfection with lysosomotropic..

    Introduction. This protocol describes a general method for transfecting mammalian cells using linear polyethylenimine. Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short term studies of protein function. We specifically use this protocol with Lenti-X 293T cells. Hours after transfection reaction has been applied to cells, gently remove media and replace with 10% glycerol or DMSO for 2-3 minutes. Remove and feed with complete media various references. *1 hour before transfection, replace cDMEM with cDMEM + 25 mM chloroquine. To understand how chloroquine CQ enhances transgene expression in polycation-based, nonviral gene delivery systems, a number of CQ analogues with variations in the aliphatic amino side chain or in the aromatic ring are synthesized and investigated. Our studies indicate that the aliphatic amino moiety of CQ is essential to provide increased gene expression.

     
  7. kseniya_www Well-Known Member

    Selected from data included with permission and copyrighted by First Databank, Inc. Probiotics and Plaquenil drug interactions - eHealthMe Protecting your eyesight when taking Plaquenil Lupus. Plaquenil and corona lupus
     
  8. S0-remont_ Well-Known Member

    Note: For a listing of dosage forms and brand names by country availability, see Dosage Forms section(s). Accepted Malaria (prophylaxis and treatment)—Hydroxychloroquine is indicated in the suppressive treatment and the treatment of acute attacks of malaria caused by Plasmodium vivax, P. In these species, “hypnozoites”, which remain dormant in the liver, are responsible for relapses. Pharmacology/Pharmacokinetics Note: Because hydroxychloroquine concentrates in the cellular fraction of blood, hydroxychloroquine concentrations measured in the blood are higher than those measured in the plasma Mechanism of action/Effect: Antiprotozoal—Malaria: Unknown, but may be based on ability of hydroxychloroquine to bind to and alter the properties of DNA. Chloroquine and hydroxychloroquine – Knowledge for medical. Hydroxychloroquine - Wikipedia Hydroxychloroquine Plaquenil Side Effects & Dosage for Malaria
     
  9. jinglesoon New Member

    Hydroxychloroquine - Side Effects, Dosage, Interactions. Hydroxychloroquine is used to treat or prevent malaria, a disease caused by parasites that enter the body through the bite of a mosquito. Malaria is common in areas such as Africa, South America, and Southern Asia. This medicine is not effective against all strains of malaria.

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  10. 4u3a Guest

    Hydroxychloroquine - Wikipedia The presence of hydroxychloroquine was confirmed through toxicologic tests. The patient's hypotension resolved within 4.5 hours, serum potassium stabilized in 24 hours, and tachycardia gradually decreased over 3 days. Interactions. The drug transfers into breast milk and should be used with care by pregnant or nursing mothers. citation needed

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